BIOS 242 Week 7 Lab 1; Bacteria of Digestive System
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$20.00
| Institution | BIOS 242 Fundamentals of Microbiology with Lab - Chamberlain |
| Contributor | Anika Fultz |
Lab 13: Bacteria of the Digestive System
Learning Objectives
- Identify how selective and differential media can be used in the identification of bacteria that can inhabit the digestive tract.
- Identify the growth of samples of presumed fecal bacteria from rest room swabs.
Introduction
The human intestinal tract is inhabited from birth by a variety of microorganisms acquired, at first, from the mother. Later, microbes are ingested in food and water, and from hands and objects placed in the mouth. Many of those do not survive the acidic pH of the stomach, which helps prevent infection from pathogens. However, those that do survive find the environment in the small intestine and colon more hospitable………..Continue
MaConkey agar (MAC)
Eosin Methylene Blue agar (EMB)
In a previous laboratory Carbohydrate Fermentation
Environmental Sampling (MUST be set-up as a demonstration by the instructor):
Exercise 1: Growing known bacteria on a variety of growth media
In this exercise, we will grow pure cultures of known bacteria in a variety of solid and liquid media.
Materials:
Method:
Exercise 2: Demonstration of Growth of GI bacteria on a variety of growth media by Collecting Specimen from toilet rim
Instructor will carry out the listed steps and show students the results.
Lab Report:
Purpose: Please describe in complete sentences and in your own words, the purpose of this experiment.
Observations:
Exercise 1: Growing known bacteria on a variety of growth media
Exercise 2: Demonstration of Growth of GI bacteria on a variety of growth media by Collecting Specimen from toilet rim
Questions:
- Explain the purpose of the following ingredients.
- Lactose in MacConkey agar
- Eosin and methylene blue dyes in eosin-methylene blue agar.
- Do all the bacteria samples grow on Eosin Methylene Blue and MacConkey agar? If not, why not?
- What does a metallic green sheen indicate on an Eosin Methylene Blue plate?
- What additional information did the lactose fermentation tubes provide?
- Why couldn’t we examine any anaerobic bacteria that are part of the normal digestive system?
| Instituition / Term | |
| Term | Uploaded 2023 |
| Institution | BIOS 242 Fundamentals of Microbiology with Lab - Chamberlain |
| Contributor | Anika Fultz |
