BIOS 242 Week 6 Assignment; Lab 12 0f 14 Onsite; Bacteria of Respiratory System
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$20.00
| Institution | Chamberlain |
| Contributor | Nikki |
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Lab 12: Bacteria of the Respiratory System
Introduction:
The respiratory system is divided into two parts: the upper and lower respiratory systems. The lower respiratory system is an axenic environment because of the cilia that lines the respiratory tract, alveolar macrophages and IgA antibodies found in the mucus. The upper part harbors a variety of microbiota such as Gram-negative bacteria Hemophilus, Gram-positive bacteria such as Staphylococcus aureus and diphtheroids that can cause opportunistic infections leading to infection like pneumonia and diphtheria.
Blood agar plates (BAP) mimic our respiratory system- they are differential in nature and demonstrate the different ways in which microorganisms hemolyze the red blood cells (RBCs) in the medium. The 3 different types of hemolysis seen on BAP are:
- Alpha hemolysis: Alpha or partial hemolysis is demonstrated by organisms such as Streptococcus pneumonieae, which can break down RBC only partially. The colonies are a greenish-yellow and discoloration of agar is observed on plates (think about appearance of your sputum when you are sick with throat infection).
- Beta hemolysis: In Beta or complete hemolysis bacteria like Streptococcus pyogenes completely digests RBCs creating a clear zone around the colonies.
- Gamma hemolysis: Enterococcus faecalis with gamma or no hemolysis does not digest RBC so agar is unchanged and no lysis occurs.
Materials:
Liquid cultures of B. subtilis, E. coli, S. epidermidis and S. marcescens, BAP plates, Incinerators, Metal loops……….. Continue
| Instituition / Term | |
| Term | Summer |
| Institution | Chamberlain |
| Contributor | Nikki |
